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001			233187
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008			180921s2018 xxu| s |||| 0|eng d
020			_a9781493987306
024	7		_a10.1007/978-1-4939-8730-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRecombinant Protein Expression in Mammalian Cells _bMethods and Protocols _cedited by David L. Hacker.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 311 páginas) _b76 ilustraciones, 68 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1850
505	0		_aTransient Gene Expression in Suspension HEK293-EBNA1 Cells -- Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells -- PEI-Mediated Transient Gene Expression in CHO Cells -- Stable Expression by Lentiviral Transduction of Cells -- Inducible Protein Production in 293 Cells using the piggyBac Transposon System -- Recombinant CHO Cell Pool Generation using piggyBac Transposon System -- Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression -- Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor -- CHO and HEK293 Cell Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors -- Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary Cells in Suspension -- Continuous and Integrated Expression and Purification of Recombinant Antibodies -- High Throughput Transfection of HEK293 Cells for Transient Protein Production -- Microfluidic Transfection for High-Throughput Mammalian Protein Expression -- Genome Wide High Throughput RNAi Screening for Identification of Genes Involved in Protein Production -- Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells -- Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells -- Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV) -- Considerations in the Use of Codon Optimization for Recombinant Protein Expression -- Versatile Cell-free Protein Synthesis Systems based on Chinese Hamster Ovary Cells.
520			_aThis detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins. Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
700	1		_aHacker, David L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493987290
776	0	8	_iPrinted edition: _z9781493987313
776	0	8	_iPrinted edition: _z9781493993772
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8730-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233187 _d233187