000			03474nam a22003615i 4500
001			233190
003			ES-VaUE
005			20221220020444.0
007			cr nn 008mamaa
008			181015s2018 xxu| s |||| 0|eng d
020			_a9781493987726
024	7		_a10.1007/978-1-4939-8772-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMorphogen Gradients _bMethods and Protocols _cedited by Julien Dubrulle.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 306 páginas) _b143 ilustraciones, 136 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1863
505	0		_aAnalysis of Expression Gradients of Developmental Regulators in Arabidopsis thaliana Roots -- Detection and Quantification of the Bicoid Concentration Gradient in Drosophila Embryos -- Imaging Cytonemes in Drosophila Embryos -- Measuring Dorsoventral Pattern and Morphogen Signaling Profiles in the Growing Neural Tube -- Fluorescence Correlation and Cross-Correlation Spectroscopy in Zebrafish -- FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos -- Generation of Ectopic Morphogen Gradients in the Zebrafish Blastula -- Polarizing Region Tissue Grafting in the Chick Embryo Limb Bud -- Analysis of PLETHORA Gradient Formation by Secreted Peptides during Root Development -- Live Imaging of mRNA Transcription in Drosophila Embryos -- LiveFly: A Toolbox for the Analysis of Transcription Dynamics in Live Drosophila Embryos -- Discrete-State Stochastic Modeling of Morphogen Gradient Formation -- Simulation of Morphogen and Tissue Dynamics -- Mechanisms and Measurements of Scale Invariance of Morphogen Gradients -- Scaling of Morphogenetic Patterns -- Modeling Time-Dependent Acquisition of Positional Information.
520			_aThis detailed book presents methods focusing on the visualization of morphogen gradients, the analysis of their biophysical and biological properties, and the theoretical aspects underlying their functions. The study of morphogen gradients combines biophysics, cell and developmental biology, and applied mathematics approaches to understand their formation, their biological functions, and the prediction of their behavior in space and time, and this volume serves as a practical, hands-on guide to the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and helpful, Morphogen Gradients: Methods and Protocols is an ideal collection for researchers in this vital area of study.
700	1		_aDubrulle, Julien _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493987719
776	0	8	_iPrinted edition: _z9781493987733
776	0	8	_iPrinted edition: _z9781493993888
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8772-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233190 _d233190