000			03750nam a22003495i 4500
001			233194
003			ES-VaUE
005			20221220020444.0
007			cr nn 008mamaa
008			190929s2020 xxu| s |||| 0|eng d
020			_a9781493998319
024	7		_a10.1007/978-1-4939-9831-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSingle Cell Metabolism _bMethods and Protocols _cedited by Bindesh Shrestha.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publishing _c2020
300			_a1 recurso en línea (XI, 226 páginas) _b74 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2064
505	0		_aSingle Cell Metabolomics by Mass Spectrometry -- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry -- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry -- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis -- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells -- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics -- Laser Capture Microdissection - Liquid Vortex Capture - Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells -- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging -- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population -- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry -- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry -- Compact Quantum Dots for Quantitative Cytology -- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry -- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry -- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics -- Ten Major Future Challenges in Single Cell Metabolomics.
520			_aThis volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field. .
700	1		_aShrestha, Bindesh _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493998296
776	0	8	_iPrinted edition: _z9781493998302
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9831-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233194 _d233194