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020 _a9781071601389
024 7 _a10.1007/978-1-0716-0138-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aNucleic Acid Detection and Structural Investigations
_bMethods and Protocols
_cedited by Kira Astakhova, Syeda Atia Bukhari.
250 _a1st edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XI, 270 páginas)
_b72 ilustraciones, 48 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2063
505 0 _aOptomagnetic Detection of Rolling Circle Amplification Products -- Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device -- RT-qPCR Detection of Low Copy HIV RNA with Yin-Yang Probes -- Solid-phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence -- 5'-Mono- and 5'-bis-pyrene 2'-O-methyl RNA Probes for Detection of RNA Mismatches -- Combined Assay for Detecting Autoantibodies to Nucleic Acids and Apolipoprotein H in Patients with Systemic Lupus Erythematosus -- Electrochemical Detection of dsDNA-specific Antibodies -- Sequence-defined DNA-amphiphiles for Drug Delivery: Synthesis and Self-assembly -- DNA-mediated Liposome Fusion Observed by Fluorescence Spectrometry -- Advanced Fluorescence Imaging to Distinguish Between Intracellular Fractions of Antisense Oligonucleotides -- Methodology for Subcellular Fractionation and microRNA Examination of Mitochondria, Mitochondria Associated ER Membrane (MAM), ER, and Cytosol from Human Brain -- Skeletal Muscle Injury by Electroporation - A Model to Study Degeneration/Regeneration Pathways in Muscle -- Isolation and Characterization of Muscle Derived Stem Cells from Dystrophic Mouse Models -- Universal Library Preparation Protocol for Efficient High-Throughput Sequencing Of Double-stranded RNA Viruses -- Quantitation of Molecular Pathway Activation using RNA Sequencing Data -- Molecular Pathway Analysis of Mutation Data for Biomarkers Discovery and Scoring of Target Cancer Drugs -- Oncobox Method for Scoring Efficiencies of Anticancer Drugs Based on Gene Expression Data -- Global Characterization of Circulating Nucleic Acids. .
520 _aThis book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.
700 1 _aAstakhova, Kira
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBukhari, Syeda Atia
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071601372
776 0 8 _iPrinted edition:
_z9781071601396
776 0 8 _iPrinted edition:
_z9781071601402
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0138-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2020
999 _c233195
_d233195