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008			191107s2020 xxu| s |||| 0|eng d
020			_a9781493998418
024	7		_a10.1007/978-1-4939-9841-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDiabetic Nephropathy _bMethods and Protocols _cedited by Luigi Gnudi, David A. Long.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XIII, 342 páginas) _b109 ilustraciones, 95 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2067
505	0		_aDiabetic Nephropathy: An Overview -- Modeling Podocyte Biology Using Drosophila Nephrocytes -- A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros -- Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model -- Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy -- Histological Examination of the Diabetic Kidney -- Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy -- Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy -- Transcutaneous Measurement of Glomerular Filtration Rate in Rodents -- An In Vitro Method to Analyze Glucose Uptake in Podocytes -- Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro -- Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy -- Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease -- Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy -- Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches -- Genetic Strategies to Understand Human Diabetic Nephropathy: In Silico Strategies for Molecular Data - Association Studies -- Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients -- Metabolomic and Proteomic Techniques for Establishing Biomarkers and Improving Our Understanding of Pathophysiology in Diabetic Nephropathy -- Formation of Mature Nephrons by Implantation of Human Pluripotent Stem Cell-Derived Progenitors into Mice -- Generating Mutant Renal Cell Lines Using CRISPR Technologies.
520			_aThis book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and to develop treatments for this condition.
700	1		_aGnudi, Luigi _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLong, David A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493998401
776	0	8	_iPrinted edition: _z9781493998425
776	0	8	_iPrinted edition: _z9781493998432
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9841-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233196 _d233196