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001			233197
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005			20221220020444.0
007			cr nn 008mamaa
008			191109s2020 xxu| s |||| 0|eng d
020			_a9781493998845
024	7		_a10.1007/978-1-4939-9884-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHistidine Phosphorylation _bMethods and Protocols _cedited by Claire E. Eyers.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 256 páginas) _b54 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2077
505	0		_aProtein Dynamics in Phosphoryl-transfer Signaling Mediated by Two-component Systems -- A High-throughput Strategy for Recombinant Protein Expression and Solubility Screening in Escherichia coli - A Case of Sensor Histidine Kinase -- SDS-PAGE and Dot Blot Autoradiography: Tools for Quantifying Histidine Kinase Autophosphorylation -- A Quantitative Method for the Measurement of Protein Histidine Phosphorylation -- Analysis of 1- and 3-phosphohistidine (pHis) Protein Modification using Model Enzymes Expressed in Bacteria -- Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry -- Protein Phosphohistidine Phosphatases of the HP Superfamily -- In Vitro Assays for Measuring Protein Histidine Phosphatase Activity -- Structural and Functional Characterization of Autophosphorylation in Bacterial Histidine Kinases -- Manipulation of Bacterial Signalling using Engineered Histidine Kinases -- Development of a Light-Dependent Protein Histidine Kinase -- Empirical Evidence of Cellular Histidine Phosphorylation by Immunoblotting using pHis mAbs -- Immunohistochemistry (IHC): Chromogenic Detection of 3-Phosphohistidine Proteins in Formaldehyde-Fixed, Frozen Mouse Liver Tissue Sections -- Subcellular Localisation of Histidine Phosphorylated Proteins through Indirect Immunofluorescence -- High throughput Characterisation of Histidine Phosphorylation sites using UPAX and Tandem Mass Spectrometry -- Proteome Bioinformatics Methods for Studying Histidine Phosphorylation. .
520			_aThis volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field. .
700	1		_aEyers, Claire E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493998838
776	0	8	_iPrinted edition: _z9781493998852
776	0	8	_iPrinted edition: _z9781493998869
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9884-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233197 _d233197