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008			191231s2020 xxu| s |||| 0|eng d
020			_a9781071601594
024	7		_a10.1007/978-1-0716-0159-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMetabolic Flux Analysis in Eukaryotic Cells _bMethods and Protocols _cedited by Deepak Nagrath.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XIII, 370 páginas) _b87 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2088
505	0		_aDetermination of Isotopologue and Tandem Mass Isotopologue Fractions using Gas Chromatography Chemical Ionization Time of Flight Mass Spectrometry: Methodology and Uncertainty of Measurement -- Non-Targeted Mass Isotopolome Analysis using Stable Isotope Patterns to Identify Metabolic Changes -- Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C Meabolic Flux Analysis -- Deuterium Tracing to Interrogate Compartment-Specific NAD(p)H Metabolism in Cultured Mammalian Cells -- Large-Scale Profiling of Cellular Metabolic Activities using Deep 13C Labeling Medium -- Analyzing the Metabolism of Metastases in Mice -- Robusy Analytical methods for the Accurate Quantification of the Total Biomass Composition of Mammalian Cells -- Quantifying Nitric Oxide Flux Distributions -- SWATH: A Data-Independent Tandem Mass Spectrometry Method to Quantify 13C Enrichment in Cellular Metabolites and Fragments -- Quantifying Metabolic Transfer Mediated by Extracellular Vesicles using Exo-MFA: An Integrated Empirical and COmputational Platform -- Comparative Metabolic Network Flux Analysis to Identify Differences in Cellular Metabolism -- Software Supporting a Workflow of Quantitative Dynamic Flux Maps Estimation in Central Metabolism from SIRM Experimental Data -- Inferring Metabolic Flux from Time-Course Metabolomics -- Using Metabolic Network Reconstructions to Predict Drug Targets and Off-Target Effects -- Single-Cell Digital Twins for Cancer Preclinical Investigation -- Supply and Demand Analysis of Autophagy -- Thermodynamic Approaches in Flux Analysis.
520			_aThis volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases. .
700	1		_aNagrath, Deepak _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071601587
776	0	8	_iPrinted edition: _z9781071601600
776	0	8	_iPrinted edition: _z9781071601617
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0159-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233199 _d233199