000			03708nam a22003615i 4500
001			233203
003			ES-VaUE
005			20221220020444.0
007			cr nn 008mamaa
008			200222s2020 xxu| s |||| 0|eng d
020			_a9781071602430
024	7		_a10.1007/978-1-0716-0243-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPeptide Nucleic Acids _bMethods and Protocols _cedited by Peter E. Nielsen.
250			_a3rd edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 308 páginas) _b121 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2105
505	0		_aFmoc-based Assembly of PNA Oligomers: Manual and Microwave-assisted Automated Synthesis -- A Robust Method for Preparing Optically Pure MiniPEG-Containing Gamma PNA Monomers -- Synthesis of Pyrrolidinyl PNA and Its Site-specific Labeling at Internal Positions by Click Chemistry -- Synthesis of PNA-peptide Conjugates as Functional SNARE Protein Mimetics -- Lipid-modified Peptide Nucleic Acids - Synthesis and Application to Programmable Liposome Fusion -- Facile Preparation of PNA-Peptide Conjugates with a Polar Maleimido-thioether Linkage -- PNA-Encoded Synthesis (PES) and DNA Display of Small Molecule Libraries -- Transcription Inhibition by PNA-induced R-loops -- Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA -- In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA) -- Reactive Quantum Dot-based FRET Systems for Target-catalyzed Detection of RNA -- Peptide Nucleic Acids for MicroRNAs Targeting -- Detection of Microorganisms by Fluorescence in situ Hybridization using Peptide Nucleic Acid -- PNA Antisense Targeting in Bacteria. Determination of Antibacterial Activity (MIC) of PNA-peptide Conjugates -- In vivo Administration of Splice Switching PNAs using the mdx Mouse as a Model System -- Near Infrared in vivo Whole Body Fluorescence Imaging of PNA -- Poly(lactic-co-glycolic acid) Nanoparticle Delivery of Peptide Nucleic Acids In Vivo -- Preparation of Conjugates for Affibody-based PNA-mediated Pretargeting. .
520			_aThis third volume exemplifies and illustrates exciting advances in peptide nucleic acids (PNA) chemistry, and serves as a vital complement to the first and second edition of the book. Chapters focus on in vivo properties and behavior and applications of PNA while providing contributions on both chemistry and nucleic acid recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Peptide Nucleic Acid: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
700	1		_aNielsen, Peter E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071602423
776	0	8	_iPrinted edition: _z9781071602447
776	0	8	_iPrinted edition: _z9781071602454
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0243-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233203 _d233203