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008			200326s2020 xxu| s |||| 0|eng d
020			_a9781071604717
024	7		_a10.1007/978-1-0716-0471-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aClinical and Preclinical Models for Maximizing Healthspan _bMethods and Protocols _cedited by Paul C. Guest.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XIV, 451 páginas) _b84 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2138
505	0		_aThe Impact of New Biomarkers and Drug Targets on Age-Related Disorders -- Vitamin D and Muscle Sarcopenia in Aging -- Quantitative Assays of Plasma Apolipoproteins -- Protocol for the Use of the Ketogenic Diet in Preclinical and Clinical Practice -- An In Vivo/Ex Vivo Study Design to Investigate Effects of Chronic Conditions and Therapeutic Compounds on Adipose Stem Cells in Animal Models -- Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations -- Investigating Alcohol Behavior and Physiology Using Drosophila Melanogaster -- Using Genome-Editing Tools to Develop a Novel In Situ Coincidence Reporter Assay for Screening ATAD3A Transcript Inhibitors -- Visualizing and Evaluating Cancer Cell Growth and Invasion by a Novel 3D Culture System -- Exploiting Plug-and-Play Electrochemical Biosensors to Determine the Role of FGF19 in Sorafenib-Mediated Superoxide and Nitric Oxide Production in Hepatocellular Carcinoma Cells -- Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines -- Real-Time PCR Analysis of Metabolism-Related Genes in a Long-Lived Model of C. Elegans -- Clinical Assessment of the NADome as Biomarkers for Healthy Aging -- 2D Gel Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Eye Lens to Identify Biomarkers of Age-Related Cataract -- Testing the Effects of Dietary Seafood Consumption on Depressive Symptoms -- Assays for Monitoring the Effects of Nicotinamide Supplementation on Mitochondrial Activity in Saccharomyces Cerevisiae -- Measurement of a Surrogate Biomarker for Arginine Vasopressin Secretion is Association with Physiometric and Molecular Biomarkers of Aging -- Liquid Chromatography Tandem Mass Spectrometry Analysis of Proteins Associated with Age-Related Disorders in Human Pituitary Tissue -- Coenzyme Q10 Assessment and the Establishment of a Neuronal Cell Model of CoQ10 Deficiency -- Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer -- Quantitative Analysis of DNA Methylation by Bisulfite Sequencing -- Histomorphometric Analysis of Anti-Aging Properties on Rat Skin -- Integration of qRT-PCR and Immunohistochemical Techniques for mRNA Expression and Localization of m1AChR in the Brain of Aging Rat -- Randomized Study Design to Test Effects of Vitamin D and Omega-3 Fatty Acid Supplementation as Adjuvant Therapy in Colorectal Cancer Patients -- Effect of Vitamin D Supplementation on Muscle Strength, Muscle Function, and Body Composition in Vitamin D-Deficient Middle-Aged Women -- The Association of Food Intake and Physical Activity with Body Composition, Muscle Strength, and Muscle Function in Postmenopausal Women -- Absolute Quantification of Plasma Apolipoproteins for Cardiovascular Disease Risk Prediction -- Multiplex Analysis of Circulating Hormone Levels in Rat Models of Age-Related Diseases -- Proteomic Analysis of Brain Tissue from a Chronic Model of Stress Using a Combined 2D Gel Electrophoresis and Mass Spectrometry Approach -- Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging -- Brain Proteomic Analysis on the Effects of the Antidepressant Fluoxetine -- MK-801 Treatment of Oligodendrocytes as a Cellular Model of Aging.
520			_aThis book presents a series of protocols in multiple disease areas affected by the aging process along with several methods which have shown progress in nutrient- or intervention-based approaches to maximize the healthspan. Of interest to researchers in the areas of chronic disease, gerontology, physical exercise, and nutrition as well as to clinical scientists, physicians, and pharmacologists, this volume also provides important information on disease mechanisms and novel drug targets as each protocol will be presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Clinical and Preclinical Models for Maximizing Healthspan: Methods and Protocols is an ideal guide for preclinical and clinical researchers working toward the discoveries needed to lead to a world society in which individuals are not only living longer lives but more productive and healthier ones.
700	1		_aGuest, Paul C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071604700
776	0	8	_iPrinted edition: _z9781071604724
776	0	8	_iPrinted edition: _z9781071604731
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0471-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233209 _d233209