000			03464nam a22003615i 4500
001			233214
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007			cr nn 008mamaa
008			200717s2020 xxu| s |||| 0|eng d
020			_a9781071607633
024	7		_a10.1007/978-1-0716-0763-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Nucleus _cedited by Ronald Hancock.
250			_a3rd edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 220 páginas) _b42 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2175
505	0		_aLive-Cell Imaging and Analysis of Nuclear Body Mobility -- Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell -- Visualizing Chromatin Modifications in Isolated Nuclei -- Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture -- Studying Proton Gradients Across the Nuclear Envelope -- COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes -- Genome-Wide Mapping of UV-Induced DNA Damage with CPD-Seq -- AP-Seq: A Method to Measure Apurinic Sites and Small Base Adducts Genome-Wide -- Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID -- Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions -- Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres -- Macromolecular Crowding Measurements with Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells -- Analysis of a Nuclear Intrinsically Disordered Proteome -- Timing of Cytosine Methylation on Newly-Synthesized RNA by Electron Microscopy -- The Nucleus of Intestinal Cells of the Bacterivore Nematode Caenorhabditis elegans as a Sensitive Sensor of Environmental Pollutants.
520			_aThis detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study. Like the previous editions, this book features contributions from top experts in their respective fields. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, The Nucleus, Third Edition will help students and postdocs become aware of vital available methodologies when planning their new projects.
700	1		_aHancock, Ronald _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071607626
776	0	8	_iPrinted edition: _z9781071607640
776	0	8	_iPrinted edition: _z9781071607657
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0763-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233214 _d233214