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020 _a9781071608029
024 7 _a10.1007/978-1-0716-0802-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aImmunometabolism
_bMethods and Protocols
_cedited by Suresh Mishra.
250 _a1st edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XVI, 284 páginas)
_b58 ilustraciones, 48 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2184
505 0 _aSingle-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq -- Monitoring Lactate Dynamics in Individual Macrophages with a Genetically-Encoded Probe -- The Conjugation of Antibodies for the Simultaneous Detection of Surface Proteins and Transcriptome Analysis at a Single-Cell Level -- Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling -- Proteomic Network Analysis of Polarized Macrophages -- The Purification and Characterization of Exosomes from Macrophages -- The Isolation and Characterization of T Lymphocyte Exosomes Using Mass Spectrometry -- Analysis of Immune-Tumor Cell Interactions Using a 3D Co-Culture Model -- Studying Adipocyte and Immune Cell Crosstalk Using a Co-Culture System -- A Gene Expression Analysis of M1 and M2 Polarized Macrophages -- Simultaneous, Quantitative Characterization of Protein ADP-Ribosylation and Protein Phosphorylation in Macrophages -- The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer -- Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by Assessing Their Oxygen Consumption and Extracellular Acidification -- The Evaluation of Mitochondrial Membrane Potential Using Fluorescent Dyes or a Membrane-Permeable Cation (TPP+) Electrode in Isolated Mitochondria and Intact Cells -- The Multiparametric Analysis of Mitochondrial Dynamics in T Cells from Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) -- The Measurement of Whole Body Glucose Homeostasis in Mice -- Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy -- Sex Differences in Immunometabolism: An Unexplored Area -- Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis.
520 _aThis detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism.
700 1 _aMishra, Suresh
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071608012
776 0 8 _iPrinted edition:
_z9781071608036
776 0 8 _iPrinted edition:
_z9781071608043
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0802-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2020
999 _c233220
_d233220