000			03778nam a22003735i 4500
001			233224
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007			cr nn 008mamaa
008			200818s2020 xxu| s |||| 0|eng d
020			_a9781071609088
024	7		_a10.1007/978-1-0716-0908-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDNA Cloning and Assembly _bMethods and Protocols _cedited by Sunil Chandran, Kevin W. George.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XIII, 334 páginas) _b81 ilustraciones, 69 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2205
505	0		_aAn Integrated Computer-Aided Design and Manufacturing Workflow for Synthetic Biology -- Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with j5 -- The AssemblX Toolkit for Reliable and User-friendly Multi-gene Assemblies -- High-Throughput PCR for DNA Part Generation -- High-Throughput DNA Assembly Using Yeast Homologous Recombination -- Highly-Multiplexed, Semi-Automated Nextera Next Generation Sequencing (NGS) Library Preparation -- Generation of MoClo Standard Parts Using Golden Gate Cloning -- Assembly of Multigene Constructs using the Modular Cloning System MoClo -- Hierarchical Modular DNA Assembly using MetClo -- PaperClip DNA Assembly: Reduce, Reuse, Recycle -- Phytobricks: Manual and Automated Assembly of Constructs for Engineering Plants -- Mobius Assembly -- Design and Implementation of Multi-Protein Expression Constructs and Combinatorial Libraries using Start-Stop Assembly -- BASIC - A Simple and Accurate Modular DNA Assembly Method -- Joint Universal Modular Plasmids: A Flexible Platform for Golden Gate Assembly in Any Microbial Host -- A Step-by-Step Protocol for COMPASS, a Synthetic Biology Tool for Combinatorial Gene Assembly -- SCRaMbLE-in: A Fast and Efficient Method to Diversify and Improve the Yields of Heterologous Pathways in Synthetic Yeast. .
520			_aThis volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aChandran, Sunil _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGeorge, Kevin W _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071609071
776	0	8	_iPrinted edition: _z9781071609095
776	0	8	_iPrinted edition: _z9781071609101
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0908-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233224 _d233224