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008			201031s2021 xxu| s |||| 0|eng d
020			_a9781071607756
024	7		_a10.1007/978-1-0716-0775-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein Downstream Processing _bDesign, Development, and Application of High and Low-Resolution Methods _cedited by Nikolaos E. Labrou.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XV, 511 páginas) _b127 ilustraciones, 78 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2178
505	0		_aProtein Purification Technologies -- High Throughput Process Development: I. Process Chromatography -- 3. High Throughput Process Development: II. Membrane Chromatography -- Media Selection in Ion Exchange Chromatography in a Single Microplate -- High Throughput Screening of Dye-Ligands for Chromatography -- Technical Optimizations for the High Throughput Purification of Antibodies on Automated Liquid Handlers -- Aqueous Two-Phase Systems for Clean-Up and Recovery of Enzymes from Plants and Plant-Derived Extracts -- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins -- Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments -- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview -- Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins -- Zbasic - A Purification Tag for Selective Ion-Exchange Recovery -- An Orthogonal Fusion Tag for Efficient Protein Purification -- Biomimetic Affinity Ligands for Protein Purification -- Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification -- Design of Affinity Chromatography Peptide Ligands through Combinatorial Peptide Libraries Screening -- ZCa: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification -- Macroporous Polymer Monoliths for Affinity Chromatography and Solid Phase Enzyme Processing -- Sample Displacement Batch Chromatography of Proteins -- Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3 -- Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins -- Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+ -- Antibody Aggregate Removal using a Mixed-Mode Chromatography Resin -- Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry -- Protein Structure Analysis and Validation with X-Ray Crystallography -- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes -- Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin -- G Protein-Coupled Receptor Expression and Purification -- (Hyper)thermophilic Enzymes: Production and Purification -- Screening of Recombinant Lignocellulolytic Enzymes through Rapid Plate Assays.
520			_aThis second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Second Edition is an ideal source of information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, biotechnology, and other related areas in both academia and industry. .
700	1		_aLabrou, Nikolaos E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071607749
776	0	8	_iPrinted edition: _z9781071607763
776	0	8	_iPrinted edition: _z9781071607770
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0775-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233229 _d233229