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008			210820s2021 xxu| s |||| 0|eng d
020			_a9781071616970
024	7		_a10.1007/978-1-0716-1697-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLong Non-Coding RNAs _bMethods and Protocols _cedited by Lin Zhang, Xiaowen Hu.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIII, 308 páginas) _b48 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2372
505	0		_aLncRNA pulldown Combined with Mass Spectrometry to Identify the Novel lncRNA-associated Proteins -- Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions of Long Noncoding RNAs with Canonical and Non-canonical RNA-binding Proteins -- Characterization of Long Non-coding RNA Associated Proteins by RNA-immunoprecipitation -- Isolation of Protein Complexes Associated with Long Non-Coding RNAs -- Detection of Long Noncoding RNA Expression by Real-time PCR -- Profiling Long Non-coding RNA expression using Custom-Designed Microarray -- Long Non-coding RNA Expression Profiling using Arraystar LncRNA Microarrays -- Nuclear RNA Isolation and Sequencing -- Capturing Endogenous Long Noncoding RNAs and Their Binding Proteins Using Chromatin Isolation by RNA Purification -- Purification and Structural Characterization of the Long Noncoding RNAs -- Simultaneous RNA-DNA FISH -- Highly Resolved Detection of Long Non-coding RNAs In Situ -- Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots -- Assessment of in vivo siRNA Delivery in Cancer Mouse Models -- Genetic Editing of Long Non-Coding RNA Using CRISPR/Cas9 Technology -- Characterization of Circular RNAs -- Identification of circRNA-Interacting Proteins by Affinity Pulldown -- Exploration to Circular RNA Interactomes by RNA Pull-Down Method -- Methods for Characterization of Alternative RNA Splicing -- Computational analysis of lncRNA from cDNA sequences -- Characterizing miRNA-lncRNA interplay -- Illuminating lncRNA Function through Target Prediction -- Long Noncoding RNAs: An Overview.
520			_aThis second edition provides a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Chapters guide readers through studies used to circular RNAs, RNA splicing, and findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Long Non-Coding RNAs: Methods and Protocols,Second Edition aims to ensure successful results in the further study of this vital field.
700	1		_aZhang, Lin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHu, Xiaowen _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071616963
776	0	8	_iPrinted edition: _z9781071616987
776	0	8	_iPrinted edition: _z9781071616994
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1697-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233240 _d233240