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008			180709s2018 xxu| s |||| 0|eng d
020			_a9781493985708
024	7		_a10.1007/978-1-4939-8570-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLung Innate Immunity and Inflammation _bMethods and Protocols _cedited by Scott Alper, William J. Janssen.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 433 páginas) _b91 ilustraciones, 70 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1809
505	0		_a500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy -- Overview of Innate Lung Immunity and Inflammation -- Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes -- Isolation and Characterization of Mouse Neutrophils -- Isolation and Characterization of Mouse Fibroblasts -- Isolation of Rat and Mouse Alveolar Type II Epithelial Cells.-Isolation and Characterization of Human Alveolar type II Cells -- Air-liquid Interface Culture of Human and Mouse Airway Epithelial Cells -- Isolation and Characterization of Human Lung Myeloid Cells.-Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells under Pathological Conditions -- Measuring Innate Immune Function in Mouse Mononuclear Phagocytes -- Measuring Neutrophil Bactericidal Activity -- Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches -- Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches -- Computational Analysis of RNA-seq Data from Airway Epithelial Cells for Studying Lung Disease -- Application of Proteomics in Lung Research -- Application of Metabolomics in Lung Research -- Functional Genomics in Murine Macrophages -- Assessment of Ozone-induced Lung Injury in Mice -- Lung Histological Methods -- Intravital Microscopy in the Mouse Lung -- Mouse Models of Acute Lung Injury and ARDS -- Mouse Models of Asthma -- Animal Models of Pulmonary Fibrosis -- Mouse Models of COPD -- Mouse Models of COPD -- Delivery of Therapeutics to the Lung.
520			_aThis detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be aguidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.
700	1		_aAlper, Scott _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aJanssen, William J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493985692
776	0	8	_iPrinted edition: _z9781493985715
776	0	8	_iPrinted edition: _z9781493993291
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8570-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233254 _d233254