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008			180921s2018 xxu| s |||| 0|eng d
020			_a9781493987061
024	7		_a10.1007/978-1-4939-8706-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Ubiquitin Proteasome System _bMethods and Protocols _cedited by Thibault Mayor, Gary Kleiger.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XV, 413 páginas) _b68 ilustraciones, 39 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1844
505	0		_aCharacterization of RING-Between-RING E3 Ubiquitin Transfer Mechanisms -- Single Turnover RING/U-Box E3-Mediated Lysine Discharge Assays -- Methods for NAD-Dependent Ubiquitination Catalyzed by Legionella pneumophila Effector Proteins -- Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for their Ubiquitin Ligase Partners -- Competition Assay for Measuring Deubiquitinating Enzyme Substrate Affinity -- Enzymatic Assembly of Ubiquitin Chains -- Ubiquitin-Activated Interaction Traps (UBAITs): Tools for Capturing Protein-Protein Interactions -- Generating Intracellular Modulators of E3 Ligases and Deubiquitinases from Phage-Displayed Ubiquitin Variant Libraries -- Integrated Proteogenomic Approach for Identifying Degradation Motifs in Eukaryotic Cells -- A Method to Monitor Protein Turnover by Flow Cytometry and to Screen for Factors that Control Degradation by Fluorescence-Activated Cell Sorting -- E. coli Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins -- Strategies to Trap Enzyme-Substrate Complexes that Mimic Michaelis Intermediates during E3-Mediated Ubiquitin-Like Protein Ligation -- Small-Angle X-Ray Scattering for the Study of Proteins in the Ubiquitin Pathway -- Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes -- Recombinant Expression, Unnatural Amino-Acid Incorporation, and Site-Specific Labeling of 26S Proteasomal Subcomplexes -- Native Gel Approaches in Studying Proteasome Assembly and Chaperones -- Measuring the Overall Rate of Protein Breakdown in Cells and the Contributions of the Ubiquitin-Proteasome and Autophagy-Lysosomal Pathways -- Methods to Rapidly Prepare Mammalian 26S Proteasomes for Biochemical Analysis -- Measurement of the Multiple Activities of 26S Proteasomes -- Exploring the Regulation of Proteasome Function by Subunit Phosphorylation -- Scalable In Vitro Proteasome Activity Assay -- Exploring the Rampant Expansion of Ubiquitin Proteomics -- Ubiquitin diGLY Proteomics as an Approach to Identify and Quantify the Ubiquitin-Modified Proteome -- Interpreting the Language of Polyubiquitin with Linkage-Specific Antibodies and Mass Spectrometry -- Dissecting Dynamic and Heterogeneous Proteasome Complexes Using In Vivo Cross-Linking Assisted Affinity Purification and Mass Spectrometry.
520			_aThis volume explores numerous techniques used to study the ubiquitin proteasome system. The chapters in this book are organized into five parts and cover topics such as determining the mechanisms of action for E2s, E3s, and DUB enzymes; the latest advances to study the formation of poly-ubiquitin chains as well as their linkage types; the binding partners of proteins in the UPS; methods for structure determination by x-ray crystallography, cryo electron microscopy and SAXS; screening assays to select for degrons or modulators of E3s and DUBs; proteomics approaches in the ubiquitin field and methods to study 26S proteasome function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, The Ubiquitin Proteasome System: Methods and Protocols is a valuable resource for both experienced and novice scientists who are interested in expanding their knowledge in this field.
700	1		_aMayor, Thibault _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKleiger, Gary _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493987054
776	0	8	_iPrinted edition: _z9781493987078
776	0	8	_iPrinted edition: _z9781493993710
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8706-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233257 _d233257