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001			233263
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005			20221220020448.0
007			cr nn 008mamaa
008			191004s2020 xxu| s |||| 0|eng d
020			_a9781493997848
024	7		_a10.1007/978-1-4939-9784-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTrinucleotide Repeats _bMethods and Protocols _cedited by Guy-Franck Richard.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publishing _c2020
300			_a1 recurso en línea (XI, 286 páginas) _b65 ilustraciones, 53 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2056
505	0		_aExperimenting with Trinucleotide Repeats: Facts and Technical Issues -- Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions -- Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae -- Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis -- Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae -- Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae -- Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay -- Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site -- Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells -- Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture -- The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model -- In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA -- FISH Protocol for Myotonic Dystrophy Type 1 Cells -- Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases -- Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich's Ataxia -- GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats -- Gene Therapy for Huntington's Disease Using Targeted Endonucleases.
520			_aThis detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.
700	1		_aRichard, Guy-Franck _eeditor literario _0(orcid)0000-0002-8233-3532 _1https://orcid.org/0000-0002-8233-3532 _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493997831
776	0	8	_iPrinted edition: _z9781493997855
776	0	8	_iPrinted edition: _z9781493997862
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9784-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233263 _d233263