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007			cr nn 008mamaa
008			191004s2020 xxu| s |||| 0|eng d
020			_a9781493998821
024	7		_a10.1007/978-1-4939-9882-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aType 2 Diabetes _bMethods and Protocols _cedited by Claire J. Stocker.
250			_a2nd edition 2020
264		1	_aNew York, NY _bSpringer International Publishing _c2020
300			_a1 recurso en línea (X, 300 páginas) _b50 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2076
505	0		_aSixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now? -- Practical Considerations for In Vivo Studies -- Nutritional Models of Type 2 Diabetes Mellitus -- Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes -- Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture -- Gene Expression Mining in Type 2 Diabetes Research -- Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes -- Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia -- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans -- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry -- Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation -- Quantification of Pancreatic Islets: Using Image Analysis Tools -- The Measurement of Calcium Signaling In Beta Cell Lines using Epifluorescence and Confocal Microscopy -- Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells -- Primary Adipocytes as a Model for Insulin Sensitivity -- Assessing Islet Transplantation Outcome in Mice -- The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent.
520			_aThis second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field. .
700	1		_aStocker, Claire J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493998807
776	0	8	_iPrinted edition: _z9781493998814
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9882-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233265 _d233265