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001			233267
003			ES-VaUE
005			20221220020448.0
007			cr nn 008mamaa
008			191017s2020 xxu| s |||| 0|eng d
020			_a9781493998531
024	7		_a10.1007/978-1-4939-9853-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGenotype Phenotype Coupling _bMethods and Protocols _cedited by Stefan Zielonka, Simon Krah.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XII, 445 páginas) _b86 ilustraciones, 61 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2070
505	0		_aScreening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin -- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins -- cDNA Display: A Stable and Simple Genotype-phenotype Coupling Using a Cell-free Translation System -- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution -- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display -- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library -- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties -- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens -- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning -- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display -- Selection and Characterization of Anti-idiotypic Shark Antibody Domains -- Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression -- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors -- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting -- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires -- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display -- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning -- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping -- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies -- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors -- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets -- Engineering Antibodies on the Surface of CHO Cells -- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies.
520			_aThis volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field. .
700	1		_aZielonka, Stefan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKrah, Simon _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493998524
776	0	8	_iPrinted edition: _z9781493998548
776	0	8	_iPrinted edition: _z9781493998555
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-9853-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233267 _d233267