000			04003nam a22003855i 4500
001			233270
003			ES-VaUE
005			20221220020449.0
007			cr nn 008mamaa
008			191109s2020 xxu| s |||| 0|eng d
020			_a9781071601464
024	7		_a10.1007/978-1-0716-0146-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChimeric Antigen Receptor T Cells _bDevelopment and Production _cedited by Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim, Virgínia Picanço-Castro.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 274 páginas) _b29 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2086
505	0		_aCAR-T Cells for Cancer Treatment: Current Design and Next Frontiers -- Selection of Antibody Fragments for CAR-T Cell Therapy from Phage Display Libraries -- Phase I/II Manufacture of Lentiviral Vectors under GMP in an Academic Setting -- Production of Lentivirus for the Establishment of CAR-T Cells -- Optimized Production of Lentiviral Vectors for CAR-T Cell -- Lentiviral Vectors Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells -- In vitro Transcribed (IVT)-mRNA CAR Therapy Development -- Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors -- Generation of CAR+ T Lymphocytes using the Sleeping Beauty Transposon System -- Platforms for Clinical-grade Car-T Cell Expansion -- CAR-T Cell Expansion in a Xuri Cell Expansion System W25 -- Methods and Process Optimization for Large Scale CAR T Expansion Using the G-Rex Cell Culture Platform -- Replication Competent Lentivirus Analysis of Vector Transduced T Cell Products used in Cancer Immunotherapy Clinical Trials -- Immunophenotypic Analysis of CAR-T Cells -- Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies -- Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays -- Analysis of CAR-mediated Tonic Signaling -- Generation of Tumor Cells Expressing Firefly Luciferase (fLuc) to Evaluate the Effectiveness of CAR in a Murine Model -- Analysis of Antitumor Effects of CAR-T Cells in Mice with Solid Tumors.
520			_aThis volume provides comprehensive methods from expert scientists working in the Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chimeric Antigen Receptor T Cells: Development and Production aims to be useful in the production of CAR-T cells, especially for therapeutic purposes.
700	1		_aSwiech, Kamilla _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMalmegrim, Kelen Cristina Ribeiro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPicanço-Castro, Virgínia _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071601457
776	0	8	_iPrinted edition: _z9781071601471
776	0	8	_iPrinted edition: _z9781071601488
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0146-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233270 _d233270