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008			191126s2020 xxu| s |||| 0|eng d
020			_a9781071601631
024	7		_a10.1007/978-1-0716-0163-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTargeting Enzymes for Pharmaceutical Development _bMethods and Protocols _cedited by Nikolaos E. Labrou.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 290 páginas) _b113 ilustraciones, 71 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2089
505	0		_aIn Silico Laboratory: Tools for Similarity-Based Drug Discovery -- The In Silico Fischer Lock-and-Key Model: The Combined Use of Molecular Descriptors and Docking Poses for the Repurposing of Old Drugs -- Determination of Half-Maximal Inhibitory Concentration of an Enzyme Inhibitor -- Applications of Differential Scanning Fluorometry and Related Technologies in Characterization of Protein-Ligand Interactions -- High-Throughput Differential Scanning Fluorimetry of GFP-Tagged Proteins -- Enzyme-Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism -- Measuring Small Molecule Binding to Escherichia coli AcrB by Surface Plasmon Resonance -- Systematic Screening of Viral Entry Inhibitors Using Surface Plasmon Resonance -- Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry -- Electrophoretic Mobility Shift Assays with GFP-Tagged Proteins (GFP-EMSA) -- On-Line Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors -- Enzymatic Bioautographic Methods -- High-Throughput Assessment of Metabolism-Induced Toxicity of Compounds on a 384-Pillar Plate -- Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors -- Ligand Fishing: An Approach for the Discovery Inhibitors from Complex Biological Mixtures -- HMG-CoA Reductase as Target for Drug Development -- Lipoxygenases as Targets for Drug Development -- Cholinesterase as a Target for Drug Development in Alzheimer's Disease.
520			_aThis volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals. Consisting of three sections, the book delves into recent biocomputing and bioinformatics protocols, state-of-the art modern biophysical, electrophoretic, and chromatographic methods and high-throughput screening approaches, as well as detailed protocols and examples of the inhibition analysis and evaluation of selected enzymes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Targeting Enzymes for Pharmaceutical Development: Methods and Protocols serves as a vital reference for academics and industry professionals working on expanding our understanding of the wide range of important enzyme targets. The chapter "Enzyme-Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
700	1		_aLabrou, Nikolaos E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071601624
776	0	8	_iPrinted edition: _z9781071601648
776	0	8	_iPrinted edition: _z9781071601655
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0163-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233271 _d233271