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020 _a9781071601839
024 7 _a10.1007/978-1-0716-0183-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Stem Cells
_bMethods and Protocols
_cedited by Muhammad Naseem, Thomas Dandekar.
250 _a1st edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XI, 150 páginas)
_b40 ilustraciones, 37 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2094
505 0 _aRegulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell Niches -- Bacterial Shoot Apical Meristem Inoculation Assay -- Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana using TAL-Effectors Nucleases -- Method to Study Gene Expression Patterns during de novo Root Regeneration from Arabidopsis Leaf Explants -- Labeling and Sorting of Arabidopsis SAM Cell Populations to Capture their Transcriptome Profile -- Plant Associated Microbes Alter Root Growth by Modulating Root Apical Meristem -- Live Imaging of Arabidopsis Axillary Meristems -- Molecular Modeling of the Interaction between Stem Cells Peptide and Immune Receptor in Arabidopsis -- Methods to Visualize Auxin and Cytokinins Signaling Activity in the Shoot Apical Meristem -- Analysis of Cell Division Frequency in the Root Apical Meristem of Lycophytes, Non-Seed Vascular Plants, using EdU Labeling -- Osmotic Treatment for Quantifying Cell Wall Elasticity in the Sepal of Arabidopsis thaliana -- Mapping a Transcriptome Guided Arabidopsis SAM Interactome -- 3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox -- Micropropagation of Rosaceous Species SAM Grown in Temperate Climate -- A New Perspective on Cryotherapy: Pathogen Elimination using Plant Shoot Apical Meristem via Cryogenic Techniques.
520 _aThis volume looks at the latest technologies and methods used by researchers to study mechanisms that control the aspects of plant stem cell signaling events. The topics discussed in this book cover shoot apical meristem inoculation assay; plant associated microbes that alter root growth by modulating root apical meristem; visualizing auxin and cytokinins signaling activity; 3D analysis of mitosis distribution pattern in the plant root tip with iRoCS toolbox; and micropropagation of rosaceous species SAM grown in temperate climate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Plant Stem Cells: Methods and Protocols is a useful resource for all researchers interested in learning more about this developing field.
700 1 _aNaseem, Muhammad
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aDandekar, Thomas
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071601822
776 0 8 _iPrinted edition:
_z9781071601846
776 0 8 _iPrinted edition:
_z9781071601853
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0183-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2020
999 _c233276
_d233276