000			04201nam a22003615i 4500
001			233279
003			ES-VaUE
005			20221220020449.0
007			cr nn 008mamaa
008			200113s2020 xxu| s |||| 0|eng d
020			_a9781071602669
024	7		_a10.1007/978-1-0716-0266-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aT-Cell Receptor Signaling _bMethods and Protocols _cedited by Chaohong Liu.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XII, 296 páginas) _b64 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2111
505	0		_aExploration of T Cell Diversity using Mass Cytometry -- Analysis of Transcriptional Profiling of Immune Cells at the Single-cell Level -- Simultaneous Measurement of Surface Proteins and Gene Expression from Single Cells -- A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells -- CRISPR/Cas9-based Genetic Screening to Study T Cell Function -- Preferential Expansion of CD4+Foxp3+ Regulatory T Cells (Tregs) In Vitro by Tumor Necrosis Factor -- CD4+ T Cell Differentiation in vitro -- Characterization of Immune Cell Subset Expansion in Response to Therapeutic Treatment in Mice -- Primary T Cell Transduction to Study Follicular Helper T Cell Differentiation -- In vitro Generation of Stem Cell Memory-like T Cells from Activated T Cells -- Artificial Antigen Presentosomes for T Cell Activation -- Imaging Chimeric Antigen Receptor (CAR) -triggered T Cell Activation -- Assessing the Impact of Phytochemicals on Immune Checkpoints: Implications for Cancer Immunotherapy -- Testing the Efficiency and Kinetics of Negative Selection Using Thymic Slices -- Investigating T Cell Receptor Signaling Dynamics in situ by Two-photon Microscopy of Thymocytes Expressing Genetic Reporters in Low-Density Chimeras -- Retroviral Gene Transduction into T-cell Progenitors for Analysis of T Cell Development in the Thymus -- An Integrated Strategy for Identifying Targets of Ubiquitin-mediated Degradation in CD4+ T Cells -- Radioisotope Based Protocol for Determination of Central Carbon Metabolism in T Cells -- Assessment of Immune Protective T cell Repertoire in Humans Immunized with Novel Tuberculosis Vaccines -- Studying Peripheral T Cell Homeostasis in Mice; A Concise Technical Review -- Detection, Expansion, and Isolation of Human MAIT Cells -- In vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets.
520			_aThis volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study the T cell activation, techniques to study subsets of Tcell's, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers.
700	1		_aLiu, Chaohong _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071602652
776	0	8	_iPrinted edition: _z9781071602676
776	0	8	_iPrinted edition: _z9781071602683
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0266-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233279 _d233279