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008			200615s2020 xxu| s |||| 0|eng d
020			_a9781071605998
024	7		_a10.1007/978-1-0716-0599-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCorneal Regeneration _bMethods and Protocols _cedited by Mark Ahearne.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XII, 250 páginas) _b66 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2145
505	0		_aIsolation and Culture of Corneal Stromal Stem Cells -- In vitro Expansion of Corneal Endothelial Cells for Transplantation -- Primary Culture of Cornea-Limbal Epithelial Cells In Vitro -- Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant -- A Guide to the Development of Human Cornea Organoids from Induced Pluripotent Stem Cells in Culture -- Gene Editing for Corneal Stromal Regeneration -- Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery.-The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea -- Formation of Corneal Stromal-like Assemblies using Human Corneal Fibroblasts and Macromolecular Crowding -- Preparation of Dried Amniotic Membrane for Corneal Repair -- Fabrication of Corneal Extracellular Matrix Derived Hydrogels -- Synthesis and Application of Collagens for Assembling a Corneal Implant -- Development and Validation of a 3D in vitro Model to Study the Chemotactic Behaviour of Corneal Stromal Fibroblasts -- Femtosecond Laser Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration -- The use of Animal Models to Assess Engineered Corneal Tissue -- X-ray Diffraction Imaging of Corneal Ultrastructure.
520			_aThis volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.
700	1		_aAhearne, Mark _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071605981
776	0	8	_iPrinted edition: _z9781071606001
776	0	8	_iPrinted edition: _z9781071606018
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0599-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233293 _d233293