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001			233296
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007			cr nn 008mamaa
008			200723s2020 xxu| s |||| 0|eng d
020			_a9781071607473
024	7		_a10.1007/978-1-0716-0747-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aIntestinal Stem Cells _bMethods and Protocols _cedited by Paloma Ordóñez-Morán.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XV, 350 páginas) _b77 ilustraciones, 67 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2171
505	0		_aIdentification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategy -- Immune-Mediated Specific Depletion of Intestinal Stem Cells -- Analysis of Aged Dysfunctional Intestinal Stem Cells -- Strategies for Measuring Induction of Fatty Acid Oxidation in Intestinal Stem and Progenitor Cells -- Visualization of Stem Cell Niche by the Fluorescence Lifetime Imaging Microscopy -- Generation and Quantitative Imaging of Enteroid Monolayers -- Autophagy Detection in Intestinal Stem Cells -- Single-Cell Transcriptional Profiling of the Intestinal Epithelium -- Single-Cell Studies of Intestinal Stem Cell Heterogeneity during Homeostasis and Regeneration -- Large-Scale Production of Recombinant Noggin and R-Spondin1 Proteins Required for the Maintenance of Stem Cells in Intestinal Organoid Cultures -- Primary Intestinal Epithelial Organoid Culture -- In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance -- Generation of Knock-Out Gene-Edited Human Intestinal Organoids -- Direct Lineage Reprogramming of Mouse Fibroblasts to Acquire the Identity of Fetal Intestine-Derived Progenitor Cells -- Single Molecule RNA FISH in Whole-Mount Organoids -- Specific Gene Expression in Lgr5+ Stem Cells by Using Cre-Lox Recombination -- Generating and Utilizing Murine Cas9-Expressing Intestinal Organoids for Large Scale Knockout Genetic Screening -- Mouse Model for Sporadic Mutation of Target Alleles to Understand Tumor Initiation and Progression and Stem Cell Dynamics -- Hemagglutinating Virus of Japan Envelop (HVJ-E)-Guided Gene Transfer to the Intestinal Epithelium -- An Intrasplenic Injection Model for the Study of Cancer Stem Cells Seeding Capacity -- Organoid Derivation and Orthotopic Xenotransplantation for Studying Human Intestinal Stem Cell Dynamics -- Advanced Colorectal Cancer Orthotopic Patient-Derived Xenograft Models for Cancer and Stem Cell Research -- Modeling Colorectal Cancer Progression through Orthotopic Implantation of Organoids.
520			_aThis detailed book encapsulates the most up-to-date methods of the intestinal stem cell field and provides guidance on a variety of techniques for studying intestinal stem cells properties. Beginning with a section on in vitro techniques to study different aspects of the intestinal stem cell functions by innovative imaging and functional assays, the volume continues with chapters detailing the single-cell transcriptional profiling method, the isolation of intestinal crypts to generate and establish 3D organoids, as well as different animal models of gastrointestinal cancer and examples of the use of in vivo methods for studying intestinal tumor-initiating cells or cancer stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Intestinal Stem Cells: Methods and Protocols aims to provide comprehensive and easy to follow protocols designed to be helpful to both seasoned researchers and newcomers to this dynamic field.
700	1		_aOrdóñez-Morán, Paloma _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071607466
776	0	8	_iPrinted edition: _z9781071607480
776	0	8	_iPrinted edition: _z9781071607497
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0747-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233296 _d233296