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008 200820s2021 xxu| s |||| 0|eng d
020 _a9781071606643
024 7 _a10.1007/978-1-0716-0664-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCapturing Chromosome Conformation
_bMethods and Protocols
_cedited by Beatrice Bodega, Chiara Lanzuolo.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XI, 322 páginas)
_b66 ilustraciones, 54 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2157
505 0 _aCapturing Chromosome Conformation -- The Chromosome Conformation Capture (3C) in Drosophila melanogaster -- 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture -- Analysis, Modeling, and Visualization of Chromosome Conformation Capture Experiments -- Targeted DNase Hi-C -- C-HiC: A High Resolution Method for Unbiased Chromatin Conformation Capture Targeting Small Locus -- Computational Analysis of Hi-C Data -- Profiling Chromatin Landscape at High Resolution and Throughput with 2C-ChIP -- Single-Cell DamID to Capture Contacts between DNA and the Nuclear Lamina in Individual Mammalian Cells -- Formaldehyde-Mediated Snapshot of Nuclear Architecture -- Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHOR Labeling -- Preparing Map of Chromosome Territories Distribution Frequency -- Higher-Order Chromatin Organization Using 3D DNA Fluorescent In Situ Hybridization -- 3D Immuno-DNA Fluorescence In Situ Hybridization (FISH) for Detection of HIV-1 and Cellular Genes in Primary CD4+ T Cells -- Visualization of Nuclear and Cytoplasmic Long Noncoding RNAs at Single-Cell Level by RNA-FISH -- 3D COMBO chrRNA-DNA-Immuno FISH -- An Algorithm for the Analysis of the 3D Spatial Organization of the Genome.
520 _aThis detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.
700 1 _aBodega, Beatrice
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aLanzuolo, Chiara
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071606636
776 0 8 _iPrinted edition:
_z9781071606650
776 0 8 _iPrinted edition:
_z9781071606667
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0664-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233301
_d233301