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020 _a9781071608609
024 7 _a10.1007/978-1-0716-0860-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aTesticular Germ Cell Tumors
_bMethods and Protocols
_cedited by Aditya Bagrodia, James F. Amatruda.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XII, 278 páginas)
_b49 ilustraciones, 44 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2195
505 0 _aBasic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research -- Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men -- Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection -- Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors -- Germ Cell Tumor Cell Culture Techniques -- Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops -- Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9 -- Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines -- Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines -- Production and Analysis of Human Primordial Germ Cell-Like Cells -- A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors -- Targeted Methylation Analyses: From Bisulfite Treatment to Quantification -- Integrated Analysis of Germ Cell Tumors -- Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors -- A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring -- Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors -- Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors.
520 _aThis volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field. .
700 1 _aBagrodia, Aditya
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aAmatruda, James F
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071608593
776 0 8 _iPrinted edition:
_z9781071608616
776 0 8 _iPrinted edition:
_z9781071608623
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0860-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233303
_d233303