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001			233304
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008			200827s2021 xxu| s |||| 0|eng d
020			_a9781071609286
024	7		_a10.1007/978-1-0716-0928-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPolypeptide Materials _bMethods and Protocols _cedited by Maxim G. Ryadnov.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIV, 268 páginas) _b80 ilustraciones, 66 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2208
505	0		_aA Shortcut to the Synthesis of Peptide Thioesters Using an HF-Free Approach -- SnoopLigase-Mediated Peptide-Peptide Conjugation and Purification -- Peptide Nanoparticles for Gene Packaging and Intracellular Delivery -- Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung -- FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures -- Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes -- Functional Peptide Nanocapsules Self-Assembled from b-Annulus Peptides -- Electrostatic Self-Assembly of Protein Cage Arrays -- Design and Generation of Self-Assembling Peptide Virus-Like Particles with Intrinsic GPCR Inhibitory Activity -- Imaging and 3D Reconstruction of De Novo Peptide Capsids -- Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra -- Forming Low Molecular Weight Hydrogels by Electrochemical Methods -- In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study -- Measurement of Peptide Coating Thickness and Chemical Composition Using XPS -- Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy -- Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials -- Ultramicrotomy Analysis of Peptide-Treated Cells.
520			_aThis book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.
700	1		_aRyadnov, Maxim G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071609279
776	0	8	_iPrinted edition: _z9781071609293
776	0	8	_iPrinted edition: _z9781071609309
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0928-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233304 _d233304