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008			200828s2021 xxu| s |||| 0|eng d
020			_a9781071608968
024	7		_a10.1007/978-1-0716-0896-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aReactive Oxygen Species _bMethods and Protocols _cedited by Jesús Espada.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 233 páginas) _b63 ilustraciones, 42 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2202
505	0		_aIntroduction to in silico Modeling to Study ROS Dynamics -- Assessment of ROS Production in Mitochondria of Live Cells -- Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy -- A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo Culture -- Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana -- Characterization of oxidative lipidomics and autophagy induction in Chlamydomonas reinhardtii under abiotic stress -- Fluorescence Detection of Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the Diauxic Shift -- Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa -- Measurement of Reactive Oxygen Species In Semen Samples Using Chemiluminescence -- Methods to Unravel Pathways of Reactive Oxygen Species in the Photodynamic Inactivation of Bacteria -- Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence Microscopy Approach -- EPR Spectroscopy as a Method for ROS Quantification in Skin -- Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones -- Singlet Oxygen Quantum Yield Determination Using Chemical Acceptors -- A General Method to Establish the Relative Efficiency of Different Sonosensitizers to Generate ROS for SDT -- Lipid Peroxidation Assay using BODIPY-phenylbutadiene Probes:A Methodological Overview -- Use of Fluorescent Probes to Detect ROS in Photodynamic Therapy. .
520			_aThis volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aEspada, Jesús _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071608951
776	0	8	_iPrinted edition: _z9781071608975
776	0	8	_iPrinted edition: _z9781071608982
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0896-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233305 _d233305