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020			_a9781071608104
024	7		_a10.1007/978-1-0716-0810-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLeukemia Stem Cells _bMethods and Protocols _cedited by César Cobaleda, Isidro Sánchez-García.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIII, 479 páginas) _b87 ilustraciones, 71 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2185
505	0		_aIntroduction and Classification of Leukemias -- Leukemia Stem Cells: Concept and Implications -- Leukemia Stem Cell Drug Discovery -- Analysis and Isolation of Mouse Leukemic Stem Cells -- Mass Cytometry of Hematopoietic Cells -- PCR Technology to Identify Minimal Residual Disease -- Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies -- Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology) -- Single-Cell Transcriptomic Analysis of Hematopoietic Cells -- In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics -- In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation -- Experimental Competitive Bone Marrow Transplant Assays -- Human T-ALL Xenografts -- An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts -- Leukemic Stem Cell Culture in Cytokine-Free Medium -- Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid -- Isolation, Culture, and Manipulation of Human Cord Blood Progenitors -- Lentiviral Transduction for Optimal LSC/HSC Manipulation -- Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes -- Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes -- Arrayed Molecular Barcoding of Leukemic Stem Cells -- In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis -- In Situ Hematopoietic Stem Cell Imaging -- A Genome-Editing System for Therapeutical Targeting of Stem Cells -- Method for the Generation of Induced Hematopoietic Stem Cells -- Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells -- High Content Imaging to Phenotype Human Primary and iPSC-Derived Cells -- Bioinformatic Methods to Identify Mutational Signatures in Cancer.
520			_aThe detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.
700	1		_aCobaleda, César _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSánchez-García, Isidro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071608098
776	0	8	_iPrinted edition: _z9781071608111
776	0	8	_iPrinted edition: _z9781071608128
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0810-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233310 _d233310