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008			201116s2021 xxu| s |||| 0|eng d
020			_a9781071610282
024	7		_a10.1007/978-1-0716-1028-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSkeletal Development and Repair _bMethods and Protocols _cedited by Matthew J. Hilton.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XV, 482 páginas) _b125 ilustraciones, 105 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2230
505	0		_a Overview of Skeletal Development -- Overview of Skeletal Repair (Fracture Healing and Its Assessment) -- Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research -- Generation of Closed Transverse Fractures in Small Animals -- A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes -- Surgical Induction of Posttraumatic Osteoarthritis in the Mouse -- Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton -- Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development -- Murine Limb Explant Cultures to Assess Cartilage Development -- Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair -- MicroCT for Scanning and Analysis of Mouse Bones -- Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties -- Phenotyping Intact Mouse Bones using Bone CLARITY -- Processing and Sectioning Undecalcified Murine Bone Specimens -- Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Hard Tissues Using Kawamoto's Film Method (2020) -- Demineralized Murine Skeletal Histology -- Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated With Perilacunar/ Canalicular Remodeling Of the Bone Matrix -- Cell Lineage Tracing: Co-localization of Cell Lineage Markers with a Fluorescent Reporter -- Immunofluorescent Staining of Adult Murine Paraffin-embedded Skeletal Tissue -- Detection of Hypoxic Regions in the Bone Microenvironment -- EdU-based Assay of Cell Proliferation and Stem Cell Quiescence in Skeletal Tissue Sections -- Whole-mount In Situ Hybridization in Murine Tissues -- Bone Marrow Stromal Cell Assays - In Vitro and In Vivo -- Isolation and Culture of Periosteum Derived Progenitor Cells from Mice -- Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage -- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts -- Mitochondrial Function and Metabolism of Cultured Skeletal Cells -- Radiolabeled Amino Acid Uptake Assays in Primary Bone Cells and Bone Explants -- RANKL-based Osteoclastogenic Assay from Murine Bone Marrow Cells -- Hematopoietic Stem Cell Cultures and Assays. .
520			_aThis second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.
700	1		_aHilton, Matthew J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071610275
776	0	8	_iPrinted edition: _z9781071610299
776	0	8	_iPrinted edition: _z9781071610305
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1028-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233313 _d233313