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008			201123s2021 xxu| s |||| 0|eng d
020			_a9781071610848
024	7		_a10.1007/978-1-0716-1084-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNuclear Reprogramming _bMethods and Protocols _cedited by Kejin Hu.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 306 páginas) _b73 ilustraciones, 54 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2239
505	0		_aPig Cloning Using Somatic Cell Nuclear Transfer -- Cloning of Monkeys by Somatic Cell Nuclear Transfer -- Production of Cardiomyocyte-like cells by Fibroblast Reprogramming with Defined Factors -- Production of Cardiomyocytes by microRNA-mediated Reprogramming In Optimized Reprogramming Media -- Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene Embodied Episomal Vectors for Rapid Selection of Exogenous DNA-free Cells -- Generation of Human Neurons by microRNA-mediated Direct Conversion of Dermal Fibroblasts -- Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-marked Lentiviral Vectors in the Chemically Defined Medium -- Episomal Reprogramming Of Human Peripheral Blood Mononuclear Cells Into Pluripotency -- Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood Mononuclear Cells -- Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling -- Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA -- Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators -- Reprogramming Porcine Fibroblast to EPSCs -- Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance -- Evaluate Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotency Markers -- G-banded Karyotyping of Human Pluripotent Stem Cell Cultures -- Become Competent in Generating RNA-seq Heat Maps in One Day for Novices without prior R Experience.
520			_aThis volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
700	1		_aHu, Kejin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071610831
776	0	8	_iPrinted edition: _z9781071610855
776	0	8	_iPrinted edition: _z9781071610862
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1084-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233319 _d233319