000			03698nam a22003615i 4500
001			233326
003			ES-VaUE
005			20221220020452.0
007			cr nn 008mamaa
008			210716s2021 xxu| s |||| 0|eng d
020			_a9781071616253
024	7		_a10.1007/978-1-0716-1625-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Phosphoproteomics _bMethods and Protocols _cedited by Xu Na Wu.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 241 páginas) _b29 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2358
505	0		_aPhosphorylation Site Motifs in Plant Protein Kinases and Their Substrates -- Protein Phosphorylation Response to Abiotic Stress in Plants -- Protein Phosphorylation in Plant Cell Signaling -- Phosphoproteomics Profiling of Receptor Kinase Mutants -- Phosphoproteomics Analysis of Soybean Roots Under Salinity by Using the iTRAQ Labeling Approach -- Universal Sample Preparation Workflow for Plant Phosphoproteomic Profiling -- Mapping Plant Phosphoproteome with Improved Tandem MOAC and Label-Free Quantification -- SILIA-Based 4C Quantitative PTM Proteomics -- Phosphoproteomics Analysis of Plant Root Tissue -- Plant Phosphopeptides Enrichment by Immobilized Metal Ion Affinity Chromatography -- Two-Dimensional Gel Electrophoresis and Pro-Q Diamond Phosphoprotein Stain-Based Plant Phosphoproteomics -- 2-D DIGE Combined with Pro-Q Diamond Staining for the Identification of Protein Phosphorylation for Chlamydomonas reinhardtii: A Successful Approach -- Plant Phosphopeptides Identification and Label-Free Quantification by MaxQuant and Proteome Discovery Software -- Phosphat 4.0: An Updated Arabidopsis Database for Searching Phosphorylation Sites and Kinase-Target Interactions -- Computational Phosphorylation Network Reconstruction: An Update on Methods and Resources -- The Application of an R Language-Based Platform cRacker for Phosphoproteomics Data Analysis -- Kinase Activity Assay Using Unspecific Substrate or Specific Synthetic Peptides.
520			_aThis detailed protocol book provides phosphoproteomics techniques currently developed for use in plants, as well as offering a variety of methodologies to analyze different types of plant phosphoproteomic data. Suitable protocols for every stage of the experiment, from protein isolation and phosphopeptides enrichment to large-scale data analysis, have been considered and explored. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Phosphoproteomics: Methods and Protocols serves as an ideal guide for researchers examining phosphoproteomics in the field of plant sciences.
700	1		_aWu, Xu Na _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071616246
776	0	8	_iPrinted edition: _z9781071616260
776	0	8	_iPrinted edition: _z9781071616277
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1625-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233326 _d233326