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008			210915s2021 xxu| s |||| 0|eng d
020			_a9781071617755
024	7		_a10.1007/978-1-0716-1775-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aInvariant Natural Killer T-Cells _bMethods and Protocols _cedited by Chaohong Liu.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 195 páginas) _b46 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2388
505	0		_aGenetic Analysis of iNKT Cell Development and Function -- Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells -- Retroviral Transduction of NKT Hybridoma Cells -- Methods for Studying Mouse and Human Invariant Natural Killer T-Cells -- Isolation and Detection of Murine iNKT Cells in Different Organs -- Identifying, Isolation, and Functional Use of Human Liver iNKT Cells -- Isolation and Characterization Methods of Human Invariant NKT Cells -- Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry -- Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry -- iNKT Cell Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach -- Expansion of Human iNKT Cells Ex Vivo -- The Expansion and Cytotoxicity Detection of Human iNKT Cells -- Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood -- Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6-eGFP Transgenic Mice -- In Vivo Cytotoxicity by α-GalCer-Transactivated NK Cells -- Redirecting iNKT Cell Anti-Tumor Immunity with αGalCer/CD1d-scFv Fusion Proteins -- Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles during Development.
520			_aThis detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Invariant Natural Killer T-Cells: Methods and Protocols is an ideal guide for researchers working with and studying iNKT cells, perhaps to provide a deeper knowledge of the human immune system.
700	1		_aLiu, Chaohong _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071617748
776	0	8	_iPrinted edition: _z9781071617762
776	0	8	_iPrinted edition: _z9781071617779
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1775-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233330 _d233330