000			03849nam a22003735i 4500
001			233354
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007			cr nn 008mamaa
008			150427s2015 xxu| s |||| 0|eng d
020			_a9781493925964
024	7		_a10.1007/978-1-4939-2596-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDNA Replication _bMethods and Protocols _cedited by Sonya Vengrova, Jacob Dalgaard.
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 295 páginas) _b36 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1300
505	0		_aA High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast -- Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae -- Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin -- Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors -- Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism -- Single-Molecule Observation Of Prokaryotic DNA Replication -- Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.
520			_aUpdated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments.  As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.
700	1		_aVengrova, Sonya _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDalgaard, Jacob _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493925971
776	0	8	_iPrinted edition: _z9781493925957
776	0	8	_iPrinted edition: _z9781493955916
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2596-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c233354 _d233354