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020 _a9781493974597
024 7 _a10.1007/978-1-4939-7459-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aDNA Topoisomerases
_bMethods and Protocols
_cedited by Marc Drolet.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 328 páginas)
_b67 ilustraciones, 21 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1703
505 0 _aType IA DNA Topoisomerases: A Universal Core and Multiple Activities -- Topoisomerase I and Genome Stability: The Good and The Bad -- DNA Topoisomerases as Targets for Antibacterial Agents -- DNA Supercoiling Measurement in Bacteria -- DNA Catenation Reveals the Dynamics of DNA Topology during Replication -- Mapping E. coli Topoisomerase IV Binding and Activity Sites -- The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling -- Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast -- Detection of oriC-Independent Replication in Escherichia coli Cells -- Single-Molecule Magnetic Tweezer Analysis of Topoisomerases -- Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases -- An Assay for Detecting RNA Topoisomerase Activity -- Studying TDP1 Function in DNA Repair -- Topoisomerase II Chromatin Immunoprecipitation -- Analyzing Mitotic Chromosome Structural Defects after Topoisomerase II Inhibition or Mutation -- Monitoring the DNA Topoisomerase II Checkpoint in Saccharomyces cerevisiae -- Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides -- A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons -- Fluoroquinolone-Gyrase-DNA Cleaved Complexes -- Detection of Topoisomerase Covalent Complexes in Eukaryotic Cells -- Visualization and Quantification of Topoisomerase-DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay -- Study of Plasmid-Mediated Quinolone Resistance in Bacteria.
520 _aThis volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, DNA Topoisomerases: Methods and Protoc ols is intended for specialists and non-specialists with an overview of selected hot topics in the field, and the latest protocols to study DNA topology, topoisomerases functions and inhibition by various compounds.
700 1 _aDrolet, Marc
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493974580
776 0 8 _iPrinted edition:
_z9781493974603
776 0 8 _iPrinted edition:
_z9781493984923
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7459-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c233358
_d233358