000			03338nam a22003735i 4500
001			233362
003			ES-VaUE
005			20221220020454.0
007			cr nn 008mamaa
008			180110s2018 xxu| s |||| 0|eng d
020			_a9781493975624
024	7		_a10.1007/978-1-4939-7562-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCircular RNAs _bMethods and Protocols _cedited by Christoph Dieterich, Argyris Papantonis.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIII, 220 páginas) _b50 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1724
505	0		_aDetection and Reconstruction of Circular RNAs from Transcriptomic Data -- Deep Computational Circular RNA Analytics from RNA-seq Data -- Genome-wide circRNA Profiling from RNA-seq Data -- Analysis of Circular RNAs using the Web Tool CircInteractome -- Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR -- Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization -- Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as -- A Highly-efficient Strategy for Overexpressing circRNAs -- Constructing GFP-based Reporter to study Back-splicing and Translation of Circular RNA -- Northern Blot Analysis of Circular RNAs -- Non-radioactive Northern Blot of circRNAs --  Characterization of Circular RNA Concatemers -- Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery -- Synthesis and Engineering of Circular RNAs -- Preparation of Circular RNA in vitro -- Discovering circRNA-microRNA Interactions from CLIP-Seq Data -- Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays. .
520			_aThis volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.  Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field. .
700	1		_aDieterich, Christoph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPapantonis, Argyris _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493975617
776	0	8	_iPrinted edition: _z9781493975631
776	0	8	_iPrinted edition: _z9781493985210
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7562-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233362 _d233362