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020 _a9781071606353
024 7 _a10.1007/978-1-0716-0635-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aSchistosoma mansoni
_bMethods and Protocols
_cedited by David J. Timson.
250 _a1st edition 2020
264 1 _aNew York, NY
_bSpringer International Publising
_c2020
300 _a1 recurso en línea (XI, 265 páginas)
_b59 ilustraciones, 36 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2151
505 0 _aPraziquantel - An Enigmatic, Yet Effective Drug -- Computer-aided Drug Design for the Identification of Novel Antischistosomal Compounds -- Immunofluorescent Localization of Proteins in Schistosoma mansoni -- Sensitive Fluorescence in situ hybridization on Semi-thin Sections of Adult Schistosoma mansoni using DIG-labeled RNA Probes -- Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke Schistosoma mansoni -- Labeling of the Schistosoma mansoni Tegument -- A Procedure for Analyzing the Proteomic Profile of Schistosoma mansoni Cercariae -- Proteomic Analysis of Schistosoma mansoni tegumental Proteins -- Measuring Histone Modifications in the Human Parasite Schistosoma mansoni -- Step-by-Step Bioinformatics Analysis of Schistosoma mansoni Long Non-coding RNA Sequences -- Identification of Transposable Elements in Schistosoma mansoni -- Structural and Functional Characterization of Schistosoma mansoni Cathepsin B1 -- Characterisation of Schistosoma mansoni dihydrofolate Reductase (DHFR) -- Characterisation of Schistosoma mansoni Glutamate-Gated Chloride Channels -- A Modified ELISA Method to Evaluate the Interaction of Schistosoma mansoni Proteins with Plasminogen -- Biophysical analysis of Schistosoma mansoni septins -- Crystallographic Studies of Triosephosphate Lsomerase from Schistosoma mansoni -- Luminescence-based, Low- and medium-throughput Assays for Drug Screening in Schistosoma mansoni Larval Stage -- Testing of Schistosoma mansoni Vaccine Targets.
520 _aThis volume details protocols for studying Schistosoma mansoni. Chapters guide readers through reviews on current drugs and drug discovery, methods to interrogate the cell biology of the worm, protocols to look at proteome level changes, prepare and work with specific proteins from S. mansoni, describes a drug screening, and how to test potential vaccine targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Schistosoma mansoni: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700 1 _aTimson, David J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071606346
776 0 8 _iPrinted edition:
_z9781071606360
776 0 8 _iPrinted edition:
_z9781071606377
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0635-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2020
999 _c233372
_d233372