000			04032nam a22003855i 4500
001			233378
003			ES-VaUE
005			20221220020455.0
007			cr nn 008mamaa
008			200630s2020 xxu| s |||| 0|eng d
020			_a9781071606162
024	7		_a10.1007/978-1-0716-0616-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCRISPR-Cas Methods _cedited by M. Tofazzal Islam, Pankaj K. Bhowmik, Kutubuddin A. Molla.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XIV, 278 páginas) _b67 ilustraciones, 58 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_a1. Efficient Homologous Recombination Mediated in Planta Gene Targeting by Egg-cell Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis -- 2. Rice gene knockout or down regulation through CRISPR-Cas9 -- 3. Simultaneous CRISPR/Cas-mediated Single Base Editing in Rice Plant at More than One Locus -- 4. CRISPR-Cas9 Mediated Genome Editing of the Model Grass Species Brachypodium distachyon -- 5. CRISPR/Cas9-mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora -- 6. An effective CRISPR/Cas9 technology for efficiently isolating positive transformants and transgene-free mutants in a wide range of plant species -- 7. An optimized RNA-guided Cas9 system for efficient simplex and multiplex genome editing in barley -- 8. Genome editing of mammalian cells using CRISPR/Cas: From in silico designing to in-culture validation -- 9. Cloning free (DNA-free) CRISPR/Cas9-mediated gene knockout in human liver cell line and its detection -- 10. A procedure to design guide RNA, assembly fragments, and detect mutation for genome editing in flax -- 11. Delivery of Cas9/sgRNA RNP into wheat microspores using synthetic CPP for genome editing and gene expression modulation -- 12. CRISPR- Cas9 mediated gene editing in wheat: a step by step protocol -- 13. CRISPR-Cas9 based genome editing of banana -- 14. Homology-directed transgene-free gene editing in Chlamydomonas reinhardtii.
520			_aThis volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
700	1		_aIslam, M. Tofazzal _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBhowmik, Pankaj K _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMolla, Kutubuddin A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071606155
776	0	8	_iPrinted edition: _z9781071606179
776	0	8	_iPrinted edition: _z9781071606186
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0616-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233378 _d233378