| 000 | 06635nam a22003735i 4500 | ||
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| 001 | 233379 | ||
| 003 | ES-VaUE | ||
| 005 | 20221220020455.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 200721s2020 xxu| s |||| 0|eng d | ||
| 020 | _a9781071605240 | ||
| 024 | 7 |
_a10.1007/978-1-0716-0524-0 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aIntrinsically Disordered Proteins _bMethods and Protocols _cedited by Birthe B. Kragelund, Karen Skriver. |
| 250 | _a1st edition 2020 | ||
| 264 | 1 |
_aNew York, NY _bSpringer International Publising _c2020 |
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| 300 |
_a1 recurso en línea (XVIII, 951 páginas) _b231 ilustraciones, 192 ilustraciones a color |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v2141 |
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| 505 | 0 | _aDisorder for Dummies: Functional Mutagenesis of Transient Helical Segments in Disordered Proteins -- Computational Prediction of Intrinsic Disorder in Protein Sequences with the disCoP Meta-predictor -- Computational Prediction of Disordered Protein Motifs using SLiMSuite -- How to Annotate and Submit a Short Linear Motif to the Eukaryotic Linear Motif Resource -- Analyzing the Sequences of Intrinsically Disordered Regions with CIDER and localCIDER -- Exploring Protein Intrinsic Disorder with MobiDB -- An Easy Protocol for Evolutionary Analysis of Intrinsically Disordered Proteins -- Expression and Purification of an Intrinsically Disordered Protein -- Production of Intrinsically Disordered Proteins for Biophysical Studies; Tips and Tricks -- Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tracts and Beyond -- Cell-free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy -- Structural Analyses of Intrinsically Disordered Proteins by Small-Angle X-ray Scattering -- Determining Rg of IDPs from SAXS data -- Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient NMR Measurements -- Quantitative Protein Disorder Assessment using NMR Chemical Shifts -- Determination of pKa Values in Intrinsically Disordered Proteins -- Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates -- Predicting Conformational Properties of Intrinsically Disordered Proteins from Sequence -- Enhanced Molecular Dynamics Simulations of Intrinsically Disordered Proteins -- Computational Protocol for Determining Conformational Ensembles of Intrinsically Disordered Proteins -- Computing, Analyzing and Comparing the Radius of Gyration and Hydrodynamic Radius in Conformational Ensembles of Intrinsically Disordered Proteins -- Binding Thermodynamics to Intrinsically Disordered Protein Domains -- Analysis of Multivalent IDP Interactions: Stoichiometry, Affinity, and Local Concentration Effect Measurements -- NMR Lineshape Analysis of Intrinsically Disordered Protein Interactions -- Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers -- Determining the Protective Activity of IDPs under Partial Dehydration and Freeze-thaw Conditions -- Screening Intrinsically Disordered Regions for Short Linear Binding Motifs -- Probing IDP Interactions with Membranes by Fluorescence Spectroscopy -- Protocol for Investigating the Interactions between Intrinsically Disordered Proteins and Membranes by Neutron Reflectometry -- Interactions of IDPs with Membranes Using Dark State Exchange NMR Spectroscopy -- Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance -- Measuring and Analysing Binding Kinetics of Coupled Folding and Binding Reactions under Pseudo-first Order Conditions -- Understanding Binding Induced Folding by Temperature Jump -- Determining Binding Kinetics of Intrinsically Disordered Proteins by NMR spectroscopy -- Determination of Protein Phase Diagrams by Centrifugation -- In vitro Transition Temperature Measurement of Phase Separating Proteins by Microscopy -- Walking along a Protein Phase Diagram to Determine Coexistence Points by Static Light Scattering -- Expression and Purification of Intrinsically Disordered Aβ Peptide and Setup of Reproducible Aggregation Kinetics Experiment -- Measuring Interactions between Tau and Aggregation Inducers with Single Molecule Förster Resonance Energy Transfer -- Detection of Multisite Phosphorylation of Intrinsically Disordered Proteins using Phos-tag SDS-PAGE -- Multiple Site-specific Phosphorylation of IDPs Monitored by NMR -- Detection of Multisite Phosphorylation of Intrinsically Disordered Proteins using Quantitative Mass-Spectrometry -- Targeting an Intrinsically Disordered Protein by Covalent Modification -- Recording in-cell NMR-spectra in Living Mammalian Cells -- In-cell NMR of Intrinsically Disordered Proteins in Mammalian Cells -- Analyzing IDPs in Interactomes. . | |
| 520 | _aThe edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com. Chapters "40 and 42 " are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. | ||
| 700 | 1 |
_aKragelund, Birthe B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 700 | 1 |
_aSkriver, Karen _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9781071605233 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071605257 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071605264 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0524-0 _z(usuarios Universidad Europea de Valencia) |
| 942 |
_2lcc _cLE |
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| 988 | _aSpringer_Protocols_2020 | ||
| 999 |
_c233379 _d233379 |
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