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001			233392
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008			201019s2021 xxu| s |||| 0|eng d
020			_a9781071609743
024	7		_a10.1007/978-1-0716-0974-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDevelopmental Biology of the Sea Urchin and Other Marine Invertebrates _bMethods and Protocols _cedited by David J. Carroll, Stephen A. Stricker.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIII, 309 páginas) _b75 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2219
505	0		_a1. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae) -- Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata -- The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae -- Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis -- Staining and Tracking Methods for Studying Sponge Cell Dynamics -- Microscopy Studies of Placozoans -- Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata -- Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging -- Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization -- Quantifying Cell Proliferation During Regeneration of Aquatic Worms -- In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis -- Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond -- Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation -- Transcriptomic Analysis in the Sea Anemone Nematostella vectensis -- RNA Interference on Regenerating Holothurian Gut Tissues -- ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos -- Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase -- Functional Genomic Studies of Trichoplax adhaerens Neuronal Activity -- A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna.
520			_aThis detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties. The techniques range from culturing the organisms to modifying their DNA. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, Second Edition is an ideal guide for researchers working with these versatile organisms and for furthering our understanding of fundamental biological questions.
700	1		_aCarroll, David J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aStricker, Stephen A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071609736
776	0	8	_iPrinted edition: _z9781071609750
776	0	8	_iPrinted edition: _z9781071609767
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0974-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233392 _d233392