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008			201119s2021 xxu| s |||| 0|eng d
020			_a9781071609620
024	7		_a10.1007/978-1-0716-0962-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Integrin Interactome _bMethods and Protocols _cedited by Miguel Vicente-Manzanares.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIX, 315 páginas) _b54 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2217
505	0		_aMeasurement of Integrin Activation and Conformational Changes on the Cell Surface by Soluble Ligand and Antibody Binding Assays -- Quantification of Integrin Activation and Ligation in Adherent Cells -- Multi-parametric Analysis of Focal Adhesions in Bi-Dimensional Substrates -- Focal Adhesion Isolation Assay using ECM Coated Magnetic Beads -- Functional Integrin Regulation through Interactions with Tetraspanin CD9 -- Proximity-dependent Biotinylation (BioID) of Integrin Interaction Partners -- Analyzing the Integrin Adhesome by in situ Proximity Ligation Assay -- Single-Protein Tracking to Study Protein Interactions during integrin-based Migration -- Biochemical Characterization of the Integrin Interactome -- Network Analysis of Integrin Adhesion Complexes -- Surface Patterning for the Control of Receptor Clustering and Molecular Forces of Integrin-mediated Adhesions -- Dynamics and Physics of Integrin Activation in Tumour Cells by Nano-Sized Extracellular Ligands and Electromagnetic Fields -- Genetic Instruction of Megakaryocytes and Platelets Derived from Human Induced Pluripotent Stem Cells for Studies of Integrin Regulation -- Quantitative Analysis of Integrin Trafficking -- Methods to Study Integrin Functions on Exosomes -- Functional Bioinformatics Analyses of the Matrisome and Integrin Adhesome -- Quantifying Polarized Extracellular Matrix Secretion in Cultured Endothelial Cells. .
520			_aThis volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.
700	1		_aVicente-Manzanares, Miguel _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071609613
776	0	8	_iPrinted edition: _z9781071609637
776	0	8	_iPrinted edition: _z9781071609644
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0962-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233393 _d233393