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020 _a9781071610602
024 7 _a10.1007/978-1-0716-1060-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMyeloid-Derived Suppressor Cells
_cedited by Sven Brandau, Anca Dorhoi.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XII, 220 páginas)
_b57 ilustraciones, 36 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2236
505 0 _aImmunophenotyping of Circulating Myeloid-Derived Suppressor Cells (MDSC) in the Peripheral Blood of Cancer Patients -- Phenotyping of Myeloid-Derived Suppressor Cells in Nonhuman Primates -- Isolation and Phenotyping of Splenic Myeloid-Derived Suppressor Cells in Murine Cancer Models -- Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells -- Isolation of Human Circulating Myeloid-Derived Suppressor Cells and Analysis of Their Immunosuppressive Activity -- High Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry -- Depletion and Maturation of Myeloid-Derived Suppressor Cells in Murine Cancer Models -- In Vitro Generation of Human Neutrophilic Myeloid-Derived Suppressor Cells -- Measuring Suppressive Activity and Autophagy in Myeloid-Derived Suppressor Cells -- In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function -- Analysis of Antimicrobial Activity of Monocytic Myeloid-Derived Suppressor Cells in Infection with Mycobacterium tuberculosis and Human Immunodeficiency Virus -- Isolation and Functional Characterization of Myeloid-Derived Suppressor Cells in Infections Under High Containment -- High-Dimensional Analysis of Circulating and Tissue-Derived Myeloid-Derived Suppressor Cells from Patients with Glioblastoma -- Single Cell Transcriptome Analysis Workflow for Splenic Myeloid-Derived Suppressor Cells from Murine Breast Cancer Models -- Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia -- Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting.
520 _aThis volume explores various experimental approaches that can be used to investigate myeloid-derived suppressor cells (MDSC) and can be useful to understand the characterization of these cells. The chapters in this book cover topics such as the study of MDSC in human patient samples; employing flow cytometry or magnetic enrichment; immunophenotyping using multi-parameter flow cytometry and mass cytometry; functional characterization of MDSC by biochemical, immunological, and microscopy approaches; MDSC characterization through the use of high-resolution genomics; and novel methods of high-resolution visualization of living tissue and the purification of granulocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Myeloid-Derived Suppressor Cells: Methods and Protocols is a valuable resource for novices in this field to help them start their research on MDSC, and experienced investigators looking to establish new protocols in their lab. This book is great for anyone working in the field of biology, medicine, and veterinary medicine. .
700 1 _aBrandau, Sven
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aDorhoi, Anca
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071610596
776 0 8 _iPrinted edition:
_z9781071610619
776 0 8 _iPrinted edition:
_z9781071610626
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1060-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233394
_d233394