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020 _a9781071612019
024 7 _a10.1007/978-1-0716-1201-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCrop Breeding
_bGenetic Improvement Methods
_cedited by Pasquale Tripodi.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XI, 280 páginas)
_b62 ilustraciones, 54 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2264
505 0 _aMethods of Development of Biparental Mapping Populations in Horticultural Crops -- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits -- Using Metabolomics to Assist Plant Breeding -- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications -- High Resolution Melting Analysis as a Tool for Plant Species Authentication -- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods -- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome -- Association Mapping in Plants -- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs) -- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis -- RNA Interference (RNAi) in Tomato Crop Reseserch -- Protoplast Based Method for Genome Editing in Tetraploid Potato -- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria -- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant -- Assembly of TALEN and mTALE-Act for Plant Genome Engineering -- Genome Editing to Achieve the Crop Ideotype in Tomato -- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging -- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis.
520 _aThis volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.
700 1 _aTripodi, Pasquale
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071612002
776 0 8 _iPrinted edition:
_z9781071612026
776 0 8 _iPrinted edition:
_z9781071612033
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1201-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233398
_d233398