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008			201207s2021 xxu| s |||| 0|eng d
020			_a9781071603819
024	7		_a10.1007/978-1-0716-0381-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCircadian Clocks _bMethods and Protocols _cedited by Steven A. Brown.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XXII, 342 páginas) _b64 ilustraciones, 53 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2130
505	0		_aReal-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock -- Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian Biology -- The Assessment of Circadian Rhythms within the Immune System -- Measuring Circadian Rhythms in Human Cells -- Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests -- Asking the Clock: How to Use Information from Questionnaires for Circadian Phenotyping -- Simple Kinetic Models in Molecular Chronobiology -- Searching Novel Clock Genes using RNAi-Based Screening -- Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei -- Using GRO-seq to Measure Circadian Transcription and Discover Circadian Enhancers -- Circadian Metabolomics from Breath -- A Lipidomics View of Circadian Biology -- Circadian Lipidomics: Analysis of Lipid Metabolites Around-The-Clock -- Phosphoproteome and Proteome Sample Preparation from Mouse Tissues for Circadian Analysis -- Circadian Phosphorylation of CLOCK and BMAL1 -- Fluorescence Live Imaging of Drosophila Circadian Pacemaker Neurons -- Monitoring Electrical Activity in Drosophila Circadian Output Neurons -- Light Input to the Mammalian Circadian Clock -- Acute In Vivo Multi-Electrode Recordings from the Mouse Suprachiasmatic Nucleus -- Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices -- Collection of Mouse Brain Slices for Bioluminescence Imaging of Circadian Clock Networks -- Computational Analysis of PER2::LUC Imaging Data -- Electrophysiological Approaches to Studying the Suprachiasmatic Nucleus -- Optogenetic Methods for the Study of Circadian Rhythms.
520			_aThis volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field.
700	1		_aBrown, Steven A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071603802
776	0	8	_iPrinted edition: _z9781071603826
776	0	8	_iPrinted edition: _z9781071603833
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0381-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233401 _d233401