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001			233404
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007			cr nn 008mamaa
008			201210s2021 xxu| s |||| 0|eng d
020			_a9781071611265
024	7		_a10.1007/978-1-0716-1126-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMultiprotein Complexes _bMethods and Protocols _cedited by Arnaud Poterszman.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 340 páginas) _b82 ilustraciones, 70 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2247
505	0		_aProduction of Multi-Subunit Membrane Protein Complexes -- Production of Multiprotein Complexes using the Baculovirus Expression System: Homology Based and Restriction Free Cloning Strategies for Construct Design -- Tagging Proteins with Fluorescent Reporters using the CRISPR/Cas9 System and Double Stranded DNA Donors -- Validation of the Production of Antibodies in Different Formats in the HEK 293 Transient Gene Expression System -- The Intervening Removable Affinity Tag (iRAT) System for the Production of Recombinant Antibody Fragments -- Isolation of Artificial Binding Proteins (Affimer Reagents) for Use in Molecular and Cellular Biology -- Interactions Measurements of Protein-DNA Complexes by Isothermal Titration Calorimetry (ITC) and Microscale Thermophoresis (MST) -- switchSENSE Technology for Analysis of DNA Polymerase Kinetics -- Sedimentation Velocity Methods for Characterization of Protein Heterogeneity and Protein Affinity Interactions -- Hands on Native Mass Spectrometry Analysis of Multi-Protein Complexes -- Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry -- Integrative Mass Spectrometry-Based Approaches for Modeling Macromolecular Assemblies -- Optimization of Sample Preparation for the Observation of Macromolecular Complexes by Electron (cryo-)Microscopy -- Solubilization and Stabilization of Native Membrane Proteins for Drug Discovery -- Practical Aspects of Super-Resolution Imaging and Segmentation of Macromolecular Complexes by dSTORM -- Multicolor FRET-FLIM Microscopy to Analyze Multiprotein Interactions in Live Cells -- Context-Specific and Proximity-Dependent Labeling for the Proteomic Analysis of Spatiotemporally Defined Protein Complexes with Split-BioID -- A Directed Evolution System for Lysine Deacetylases.
520			_aThis volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focuses on sample preparation, and covers strategies for recombinant expression of multiprotein complexes in prokaryotic and eukaryotic hosts, for genome engineering using the CRISPR/Cas9 system and for production of specific binders such as reformatted antibodies and artificial binding proteins. Part Two looks at the biophysical methods that can provide useful indicators for sample optimization, and often complement structural information obtained with core technologies for structure determination-x-ray crystallography and cryo-electron microscopy-by quantitative solution data. Part Three discusses the characterization of multiprotein complexes in a cellular environment using the latest technologies and in vivo approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Multiprotein Complexes: Methods and Protocols is a valuable resource for structural and molecular biologists who need to prepare multi-components for their applications, and for other scientists working on macromolecular assemblies from other angles that need to know the latest approaches that the field has to offer.
700	1		_aPoterszman, Arnaud _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071611258
776	0	8	_iPrinted edition: _z9781071611272
776	0	8	_iPrinted edition: _z9781071611289
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1126-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233404 _d233404