000			03878nam a22003735i 4500
001			233405
003			ES-VaUE
005			20221220020457.0
007			cr nn 008mamaa
008			201214s2021 xxu| s |||| 0|eng d
020			_a9781071611197
024	7		_a10.1007/978-1-0716-1119-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChondrocytes _bMethods and Protocols _cedited by Tariq M. Haqqi, Véronique Lefebvre.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIII, 229 páginas) _b46 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2245
505	0		_aIsolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D -- Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage -- Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse -- Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures -- Fetal Growth Plate Cartilage: Histological and Immunohistochemical Techniques -- Preparation of Adult Mouse Skeletal Tissue Sections for RNA In Situ Hybridization -- MicroRNA In Situ Hybridization in Paraffin-Embedded Human Articular Cartilage and Mouse Knee Joints -- Laser Capture Microdissection of Mouse Growth Plate Cartilage -- RNA Isolation from Articular Cartilage Tissue -- Small Nucleolar RNA Expression Profiling in Cartilage -- MicroRNA Expression Profiling, Target Identification, and Validation in Chondrocytes -- ChIP-Seq Assays from Mammalian Cartilage and Chondrocytes -- MicroRNAs and Regulation of Autophagy in Chondrocytes -- Mitochondrial Biogenesis, Activity, and DNA Isolation in Chondrocytes -- Assessing Chondrocyte Status by Immunofluorescence-Mediated Localization of Parkin Relative to Mitochondria.
520			_aThis detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix. Divided into three parts, the volume explores experimental models to study chondrocytes, in vivo assays, as well as methods to characterize the chondrocyte phenotype with high-throughput or discrete assays on cells maintained in vivo, just isolated from their tissue (ex vivo), or cultured in vitro. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chondrocytes: Methods and Protocols is an ideal guide for experts as well as newcomers in the cartilage field with the aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments.
700	1		_aHaqqi, Tariq M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLefebvre, Véronique _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071611180
776	0	8	_iPrinted edition: _z9781071611203
776	0	8	_iPrinted edition: _z9781071611210
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1119-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233405 _d233405