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008			201214s2021 xxu| s |||| 0|eng d
020			_a9781071611623
024	7		_a10.1007/978-1-0716-1162-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDetection of Cell Death Mechanisms _bMethods and Protocols _cedited by Ayesha B. Alvero, Gil G. Mor.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 265 páginas) _b66 ilustraciones, 60 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2255
505	0		_aDetermination of Caspase Activation by Western Blot -- Detection of Unfolded Protein Response by Polymerase Chain Reaction -- Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway -- A Triple-Parameter Based Laboratory Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells -- Flow Cytometric Analyses of p53-mediated Cell Cycle Arrest and Apoptosis in Cancer Cells -- A Real-Time, Bioluminescent Apoptosis Assay -- Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity -- Time and Dose Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay -- Visualization and Quantification of Neutrophil Extracellular Traps -- 2In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps -- Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture -- Visualization of Necroptotic Cell Death through Transmission Electron Microscopy -- Methodology for Comprehensive Detection of Pyroptosis -- Determination and Quantitation of Cytotoxic T Cell-Mediated Cell Death -- Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model -- A Luminescent Assay to Quantify Cell Viability in Real Time -- Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis -- Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes -- Determining Cell Death Pathway and Regulation by Enrichment Analysis -- Transcription Factor Binding Site Identification and Enrichment Analysis.
520			_aThis volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.
700	1		_aAlvero, Ayesha B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMor, Gil G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071611616
776	0	8	_iPrinted edition: _z9781071611630
776	0	8	_iPrinted edition: _z9781071611647
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1162-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233407 _d233407