000			04461nam a22003855i 4500
001			233446
003			ES-VaUE
005			20221220020500.0
007			cr nn 008mamaa
008			170608s2017 gw | s |||| 0|eng d
020			_a9783662531082
024	7		_a10.1007/978-3-662-53108-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHydrocarbon and Lipid Microbiology Protocols _bMeso- and Microcosms _cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
250			_a1st edition 2017
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2017
300			_a1 recurso en línea (X, 173 páginas) _b40 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIntroduction -- Preparing the hydrocarbon / crude oil -- High pressure cultivation of hydrocarbonoclastic aerobic bacteria -- In vitro high pressure incubation and activity measurement of deep-sea, methanogenic archaea -- Bioreactor design to emulate deep-sea hydrocarbon releases including formation of gas hydrates -- Mudflat benthic spill simulations -- Protocols for subtidal and deep sea benthic oil spill simulations -- Simulation of anoxic/oxic oscillations in crude oil-degrading bioreactors -- Protocols for microcosms for growing biofilms on hydrophobic substrates - a polyphasic approach to study biodiversity, metabolic activity and biofilm architecture -- Application of micro- and mesocosms experiments to pollutant effects in biofilms -- Mini sediment columns and two-dimensional sediment flow-through microcosms - versatile model systems for studying biodegradation of organic contaminants in groundwater ecosystems.
520			_aThis Volume describes methods for simulating natural environments by using reproducible and controllable meso- and microcosm experiments to analyse hydrocarbon-degrading microorganisms and to test hypotheses. It presents important aspects of the preparation of experimental set-ups, hydrocarbon application and sampling, and features protocols for experiments with different types of samples, such as biofilms, aquatic systems (e.g. groundwater, streams), and sediments, including oscillating oxic-anoxic mesocosms, as well as for in-situ experimentation in subtidal and deep sediments. Two chapters are dedicated to cultivation under high-pressure conditions, and several chapters include protocols for processing samples for downstream chemical, microbial or activity analyses. Several of the approaches presented are generic and will benefit anyone embarking on designing meso- and microcosm experiments. < Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
700	1		_aMcGenity, Terry J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTimmis, Kenneth N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNogales, Balbina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783662531075
776	0	8	_iPrinted edition: _z9783662531099
776	0	8	_iPrinted edition: _z9783662571125
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-53108-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233446 _d233446