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001			233448
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007			cr nn 008mamaa
008			150124s2015 xxu| s |||| 0|eng d
020			_a9781493922697
024	7		_a10.1007/978-1-4939-2269-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChemical Biology _bMethods and Protocols _cedited by Jonathan E. Hempel, Charles H. Williams, Charles C. Hong.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIV, 333 páginas) _b65 ilustraciones, 40 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1263
505	0		_aIdentification of Therapeutic Small Molecule Leads in Cultured Cells Using Multiplexed Pathway Reporter Read-outs -- Applying the Logic of Genetic Interaction to Discover Small Molecules That Functionally Interact with Human Disease Alleles -- Construction and Application of a Photo-cross-linked Chemical Array -- High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem Cells -- Small Molecule High-Throughput Screening Utilizing Xenopus Egg Extract -- Fission Yeast-based High-throughput Screens for PKA Pathway Inhibitors and Activators -- A Method for High-throughput Analysis of Chronological Aging in Schizosaccharomyces pombe -- Protocols for the Routine Screening of Drug Sensitivity in the Human Parasite Trichomonas vaginalis -- Chemical Genetic Screens Using Arabidopsis thaliana Seedlings Grown on Solid Medium -- Small Molecule Screening Using Drosophila Models of Human Neurological Disorders -- High-throughput Small Molecule Screening in Caenorhabditis elegans -- Whole-organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish -- High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in Zebrafish -- Extraction Methods of Natural Products from Traditional Chinese Medicines -- Bioassay-guided Identification of Bioactive Molecules from Traditional Chinese Medicines -- NMR Screening in Fragment-Based Drug Design: A Practical Guide -- Practical Strategies for Small Molecule Probe Development in Chemical Biology -- Principal Component Analysis as a Tool for Library Design: A Case Study Investigating Natural Products, Brand-name Drugs, Natural Product-like Libraries, and Drug-like Libraries -- Small Molecule Library Screening by Docking with PyRx -- Fluorous Photoaffinity Labeling to Probe Protein-Small Molecule Interactions -- Identification of the Targets of Biologically Active Small Molecules Using Quantitative Proteomics -- Drug Affinity Responsive Target Stability (DARTS) for Small Molecule Target Identification -- Chemical Genomic Profiling via Barcode Sequencing to Predict Compound Mode of Action -- Image-Based Prediction of Drug Target in Yeast.
520			_aThis volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organized into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.
700	1		_aHempel, Jonathan E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWilliams, Charles H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHong, Charles C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493922703
776	0	8	_iPrinted edition: _z9781493922680
776	0	8	_iPrinted edition: _z9781493952847
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2269-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c233448 _d233448